Mikey rescues Stuart, a stray cat, who wreaks havoc at Third Street School. When the gang loses Stuart, he is found by the Ashleys, thus starting a fight between the two groups over ownership of the cat.
- The two men from animal control resemble Horace and Jasper Baddun from One Hundred and One Dalmatians.
- This episode was part of a special "Stuart-themed" Disney's One Saturday Morning block, where all three main shows from the block premiered episodes with a character named "Stuart" in them. Aside from "Operation Stuart", Disney's Doug had "Quailman vs. The Annoying S.T.U.A.R.T.", while Pepper Ann had "Presenting Stewart Waldinger/P.A.'s Life in a Nutshell".
- This was the only episode written by Katy Ballard.
- This episode is similar to the Doug episode "Doug's Fat Cat", where Doug has to take care of Roger's pet cat, Stinky, to find out "he" is a "she" after having kittens.
- In the Latin Spanish dub, Stuart is named "Sindulfo", which was later renamed "Sindulfa".
- T.J. Detweiler - Andrew Lawrence
- Vince LaSalle - Rickey D'Shon Collins
- Ashley Spinelli/Kindergartner #2 - Pamela Segall-Adlon
- Gretchen Grundler - Ashley Johnson
- Mikey Blumberg/Tubby/Kid #1 - Jason Davis
- Gus Griswald - Courtland Mead
- Miss Finster - April Winchell
- Miss Grotke - Allyce Beasley
- Principal Prickly - Dabney Coleman
- Guru Kid/Sam - Klee Bragger
- Dave - Ryan O'Donohue
- Captain Sticky/Kindergartner #1 - E.G. Daily
- Ashley Armbruster - Anndi McAfee
- Ashley Boulet - Francesca Smith
- Ashley Quinlan - Rachel Crane
- Hustler Kid - Michael Shulman
- Hank - Paul Dooley
- Miss Lemon - Tress MacNeille
- Stuart/Kittens - Frank Welker
- Animal Control Member - Gregg Berger
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